Figure 3

Curcumin induced apoptosis through activation of caspase-3 followed by PARP degradation. KG1a, Kasumi-1 and U937 cells were incubated with indicated concentrations of curcumin for 24 h. (A) Cells were stained with Wright-Giemsa and then examined under a light microscope. Arrows indicate apoptotic cells (magnification ×400). (B) Cells were stained with Annexin V/PI to analyze apoptotic cell populations. The graph displays the means ± SD of four independent experiments. (C) Western blotting analysis showed cleaved caspase-3 (17, 19 kDa) and cleaved PARP (89 kDa) fragment. Three independent experiments were performed with similar results, and representative data are shown.