Release of CXCL-10 by CD14+ monocytes exposed to IFNα in vivo. Release of CXCL-10 by CD14+ monocytes exposed to IFNα in vivo. The levels of CXCL-10 were measured in the supernatants of monocytes isolated from PBMC collected 24 hours after treatment from subjects receiving placebo (white bar), 1 (grey bars) or 3 (black bars) MU of IFNα + HBV vaccine. Monocytes isolated from 5-6 subjects per group were sensitized in vitro with the HBV specific antigen HBsAg (A) or left untreated (B) as described in Methods section. Red dotted line: Mean, Black line: Median, Box: 25th to 75th percentile, whiskers:10th to 90th percentile. * p < 0.05 (Wilcoxon Matched Pairs test).