Immunophenotipic analysis of PBMC of subjects treated with IFNα. Effects of IFNα administration on PBMC subsets phenotype in vivo, analyzed by FACS analysis on PBMC obtained from healthy donors undergoing HBV vaccine plus IFNα administration. Blood samples were drawn before (T0, T1m) and 24 h after (T0+24, T1m+24) the first and the second vaccine administration and cells were isolated by ficoll gradient-centrifugation and labeled with specific antibodies, as described in Methods section. For each subset, the most appropriate parameter (% of total PBMC; index = CD14+CD40+/CD14+CD40- or CD14+CD16+CD40+/CD14+CD16+CD40- ratio; MFI = mean fluorescence intensity) is shown for the average of n = 10 samples per group. * p < 0.05 (Wilcoxon Matched Pairs test).