Functional classification of genes modulated by IFNα in vivo. The list of transcripts significantly modulated by IFNα in vivo was selected by matching the lists generated by Class comparison between all "pre" vs "post" treatment samples in melanoma patients and healthy subjects receiving IFNα as vaccine adjuvant (487 and 311 respectively). The gene lists were matched, and the 130 genes consistently up-regulated (A) and the 34 consistently down-regulated (B) were separately analyzed for Gene Ontology by means of David (Biological Process, ALL levels). Biological process classes were ranked according to the percentage of the genes of the lists fitting each class (blue) in proportion to the global composition of the array (light purple). The p value of the modified Fisher test classes enrichment (p < 0.05) is shown.