Skip to main content
Figure 3 | Journal of Translational Medicine

Figure 3

From: Inhibition of phosphorylated c-Met in rhabdomyosarcoma cell lines by a small molecule inhibitor SU11274

Figure 3

Inhibition effect of SU11274 on proliferation and intracellular signaling in RMS cells. A and B, Cells were plated in 96-well plates and allowed to adhere overnight followed by treatment with the indicated concentrations of SU11274 without (A) or with (B) 10 ng/ml HGF. MTT proliferation/viability assay was performed after 72 h treatment. Data represent mean ± SD for triplicate independent experiments. C and D, expression of c-Met (C) and its downstream kinases (D) modulated in three RMS cell lines after treatment with 5 μM SU11274 for 24 h. RMS cells were pre-starved and stimulated with 10 ng/ml of HGF for 7.5 min. Cells were harvested and immunoblotted using phospho-specific antibodies against phospho-c-Met (pY1234/1235), phospho-STAT3 (Tyr705), phospho-AKT (S473) and phospho-ERK1/2 (T202/204). *short exposure; **long exposure.

Back to article page