Table 1 SRM transitions and collision energies used in LC-MS/MS for the detection of BBR, M1, M2, M3 and M4
From: Bioactivities of berberine metabolites after transformation through CYP450 isoenzymes
Chemical | Molecular mass (MW) | SRM transition (m/z) | Collision energy (eV) |
|---|---|---|---|
BBR | 335 | 336.0→319.8 | 28 |
M1 | 321 | 322.0→306.8 | 31 |
M2 | 321 | 322.0→306.8 | 31 |
M3 | 323 | 324.0→307.8 | 32 |
M4 | 338 | 338.0→321.9 | 30 |