Figure 6

The adhesion of polymorphonuclear neutrophils (PMN). The adhesion was measured 24 hours after treatment with pyrrolidine dithiocarbamate (PDTC) at different concentrations and challenges with leukotriene B4 (LTB4), interleukin-8 (IL-8) and lipopolysaccharide (LPS). Levels of IL-8 in the supernatant of PMN culture were measured 0, 3, 6, 9, 12, 18 and 24 hours after the challenge with LPS or vehicle and treatment with vehicle, PDTC alone, wortmannin (WT) alone or the combination of PDTC and WT.