Activities of nuclear factor kappa B (NF-κB) in polymorphonuclear neutrophils (PMN). Activities were calculated as referred to the average value of PMN NF-κB activities before the intravenous challenge and treatment. PMNs were isolated from animals treated and challenged with vehicle (A), treated with vehicle and challenged with lipopolysaccharide (LPS) (B), or treated with pyrrolidine dithiocarbamate (PDTC) and challenged with LPS. Animals were intravenously challenged and treated for 0 (before challenge), 1, 2, 4 and 6 hours and each group had 20 animals. Representatives of the electrophoretic mobility shift assay of NF-κB activation in PMN were also shown.