Figure 4

Effect of resveratrol on hepatic sinusoidal endothelial cell response to tumor-derived factors. A) Representative experiment on the effect of RVL and IL-18 on VCAM-1 protein expression by tumor-activated HSE. Freshly isolated and primary cultured murine HSE cells were incubated for 30 min with 2.5 μM RVL or vehicle, and next with B16M-CM or 1 ng/ml IL-18 overnight. Then, cell lysates were obtained and VCAM-1 protein was analyzed by Western Blot. Quantification of VCAM-1 expression was normalized to that of β-tubulin. B) Effect of RVL on B16M cell adhesion to untreated and B16-CM treated HSE in vitro. Cultured HSE were incubated with basal medium and B16M-CM for 6 hours. In some wells, 2.5 μM RVL was added 30 minutes before B16M-CM. Then, cell adhesion assay was performed as described in Methods. The results are average values ± SD of 3 separated experiments, each in triplicate (n = 9). Differences in the percentage of adhering cells with respect to untreated HSE (*) or B16M-CM treated ESH (**) were statistically significant (P < 0.01) by ANOVA and Bonferoni's post-hoc test.