Figure 9
Immunofluorescent staining for renal heme oxygenase (HO)-1 and alpha-smooth muscle actin (α-SMA) expressions after ischemia-reperfusion (IR). Remarkably reduced number of HO-1-positive cells (green arrows) in interstitial and peri-tubular area of kidney in (A) Control group, than in (B) IR group, and (C) IR + ADMSC group. (D) Notably lower number of HO-positive cells in IR group than in IR + ADMSC group. *p < 0.02 between indicated groups. Scale bars in right lower corners represent 50 μm (200 ×) (Upper panel). Alpha-SMA staining showing notably higher number of small vessel (defined as diameter <25 μm) (yellow arrows) in (E) Normal controls, than in (F) IR group, and (G) IR + ADMSC group. (H) Significantly higher number of α-SMA-positive cells in IR + ADMSC group than in IR group. *p < 0.02 between indicated groups. Scale bars at right lower corner represent 100 μm (100 ×) (Lower panel). (n = 8 for each group)