Table 1 Primer and oligo sequences for PCR, ribozyme and amplification of full coding sequence of prostate transglutaminase (TGase-4)
| Â | Sense (5' -'3) | AntiSense (5' - '3) |
|---|---|---|
TGase-4 expression | Atgatggatgcatcaaaaga | Ctacttggtgatgagaacaatcttctga |
TGase-4 (position 62) | Atggatgcatcaaaagagc | Aggtgaaacacctgtcctc (Aactgaacctgaccgtacaa ggtgaaacacctgtcctc [for Q-PCR]) |
TGase-4 (position 1957) | Ataaaatgcaccccaataaa | Ctacttggtgatgagaacaatc (Actgaacctgaccgtacacc tacttggtgatgagaacaatc [for Q-PCR]) |
GAPDH | Agcttgtcatcaatggaaat | Cttcaccaccttcttgatgt |
GAPDH for Q-PCR | Ctgagtacgtcgtggagtc | Actgaacctgaccgtacacagagatgatgacccttttg |