Staining of MDA-7 in PC-3 cells (A) and co-localization of TGase-4 and MDA-7/MDA-7 receptor in human prostate tissues (B, C and D). A: Staining of MDA-7 in wild type (top left), control transfected cells (bottom left) and in TGase-4 expression vector transfected PC-3 cells (right two micrographs). PC-3 stained positive for MDA-7. However, after over-expressing TGase-4, staining intensity of MDA-7 is reduced. B and D: co-localization of TGase-4 and MDA-7 in normal (B) and tumour (D) prostate tissues. TGase-4 staining appears in both stroma and in the cells. C: co-localization of TGase-4 and MDA-7 receptor, IL20R, in prostate tissue. TGase-4 and IL20R have a good degree of co-localization. HOE: Nuclear staining using Hoescht 33258. Comp.: Double immunofluorescent staining reaction obtained with each of the respective antibodies in B, C and D. Magnification was ×100.