Figure 5
From: High throughput RNAi assay optimization using adherent cell cytometry
Comparison of siGLO Red counts per cell in different treatment conditions. (A) Comparison of siGLO Red pixel counts/cell among different siRNA treatment conditions in AoSMCs that have been transfected without transfection reagent, or with HiPerfect, or RNAiMax. (B) Transposition of (A). Comparison of siGLO Red pixel count/cell among different transfection reagents in AoSMCs that have either not been transfected or were transfected with 50 nM control siRNA, 5 nM siGLO Red or 50 nM siGLO Red.