Figure 4

Comparison of transfection reagents in siGLO Red transfected AoSMCs. (A, D, and G) No transfection reagent with 50 nM control siRNA, 5 nM siGLO Red and 50 nM siGLO Red respectively. (B, E, and H) HiPerfect transfection reagent (0.375 μl/100 μl) with 50 nM control siRNA, 5 nM siGLO Red and 50 nM siGLO Red respectively. (C, F, and I) RNAiMax transfection reagent (0.375 μl/100 μl) with 50 nM control siRNA, 5 nM siGLO Red and 50 nM siGLO Red respectively. (J) Representativepicture of 50 nM siGLO Red + RNAiMax transfected AoSMCs used for quantification. AoSMCs were also stained with Hoechst nuclei stain. Both fluorescent signals were confined within the cells. (K) Represents Figure 4J with the gated fluorescent events (red dots/circles = siGLO Red; blue circles = Hoechst stained nuclei). Note the exact overlay of actual and detected fluorescent events. The vast majority of gated signals originated from the AoSMCs.