Scatter plots of control siRNA or siGLO Red transfected AoSMCs using different transfection reagents. Composition of scatter plot diagrams generated with Celigo® system software. Parameters depicted are fluorescence area (x-axis) and integrated fluorescence intensity (y-axis). (A) 50 nM unlabeled control siRNA, no transfection reagent group. The area boxed in yellow represents the majority of red signal fluorescence (background fluorescence) in this group. (B) 50 nM siGLO Red, no transfection reagent group. The yellow highlighted area from (A) serves as a comparison. (C) 50 nM siGLO Red with HiPerfect group. Boxed area of (A) compared to the gated events recorded in (C). (D) 50 nM siGLO Red with RNAiMax group. Area of (A) compared to gated events recorded in AoSMCs transfected with 50 nM siGLO Red complexed with RNAiMax transfection reagent.