Figure 2

Surface antigen profiling. HuES9.E1 and E1-MYC 16.3 MSCs were stained with an appropriate antibody conjugated to a fluorescent dye and analyzed by FACS. The fluorescence of HuES9.E1 or E1-MYC 16.3 was the average cellular fluorescence of cells at p16 or p6. Nonspecific fluorescence was assessed by incubating the cells with isotype-matched mouse monoclonal antibodies.