Figure 2
Transduction of adenoviruses in LLC cells in vitro. A, adenoviral gene transduction efficiencies in murine LLC and human A549 cell lines at indicated MOIs. B, HRP expression of LLC cells in various treatment groups was analyzed by western blot. C, IL-12 expression in the supernatant of LLC cells infected with AdCMV(-), AdhTERTHRP and/or AdCMVmIL-12 was quantified using mIL-12p70 ELISA kit following the manufacturer's instructions. N.D., no detection. D, effects of transduction with different adenoviruses followed by IAA treatment in LLC cells in vitro. Transduced cells were incubated with various concentrations of IAA for 5 days, and cell viability was determined by MTT assay. IC50 was calculated as the concentration of drug which inhibited cell growth by 50%. Data were representative of three independent experiments. Each point represented the means ± SEM and was expressed as percentage relative to drug-free cells.