HDAC inhibitor inhibits MMPs expression activated by IL-13 through induction of IL-13Rα2. A, Conventional RT-PCR for expression of MMPs was performed after cells were incubated with 1 μM TSA and/or 10 μM SP600125 for 24 hours. Twenty-two hours prior to harvesting cells, IL-13 was added to the cultured medium and total RNA extracted. β-actin is shown as a reference gene. B, MMPs expression in IL-13Rα2 knock-down (α2KD) cells incubated with TSA. Mock and α2KD cells were treated with TSA and IL-13 same as in panel B.