Modulation of IL-13Rα2 protein by HDAC and AP-1 inhibitors and stability of IL-13Rα2 expression. A, ICC of IL-13Rα2 after incubation with TSA and SP600125 is shown. Cells were incubated with 1 μM TSA and/or 10 μM SP600125 for 24 hours and fixed by 4% PFA. IL-13Rα2 was visualized by Alexa488. Recovery of IL-13Rα2 expression after incubation with TSA (B) and SP600125 (C). Cells were incubated with 1 μM TSA or SP600125 for 24 hours or 12 hours, respectively and then inhibitors were removed by replacing with new medium without TSA for 1-5 days or SP600125 for 12-48 hours. IL-13Rα2 gene expression was determined by conventional RT-PCR.