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Figure 2 | Journal of Translational Medicine

Figure 2

From: Histone modification enhances the effectiveness of IL-13 receptor targeted immunotoxin in murine models of human pancreatic cancer

Figure 2

Regulation of IL-13Rα2 expression by HDAC and AP-1 inhibitors. A, Conventional RT-PCR of IL-13Rα2 mRNA after incubation with TSA. Cells were incubated with 1 or 5 μM TSA for 24 hours and total RNA was extracted. PM-RCC cells were used as a positive control. β-actin is shown as a reference gene. B, Conventional RT-PCR of IL-13Rα2 after incubation with NaB. Cells were incubated with 0 - 50 mM NaB for 24 hours and total RNA extracted. C, Conventional RT-PCR of IL-13Rα2 gene after incubation with SP600125. Cells were incubated with 10 μM SP600125 for 6 or 12 hours and total RNA extracted. D, Conventional RT-PCR of IL-13Rα2 after incubation with AP-1 inhibitor, SR11302. Cells were incubated with 0 - 100 μM SR11302 for 12 hours and total RNA extracted.

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