IL-13Rα2 expression in pancreatic cancer and normal cell lines and DNA methylation and Histone modification of IL-13Rα2 promoter. A, qRT-PCR for IL-13Rα2 expression in pancreatic cancer and normal cell lines was performed. Data shown is ratio of human IL-13Rα2/β-actin expression and multiplied by 222 for convenience. Bars, SD of triplicate determinations. B, Bisulfite-sequencing of IL-13Rα2 promoter. Only one CpG site is present within the IL-13Rα2 promoter region. Methylated and unmethylated alleles are shown as solid and open circles, respectively. C, Acetylation and methylation status of histones H3 and H4 in pancreatic cancer and normal cell lines. The region around the IL-13Rα2 promoter was amplified by qPCR after ChIP using anti-acetylated histone H3 and H4 antibody and anti-methylated H3K9. Results were standardized by amplification of the IL-13Rα2 promoter using DNA before precipitation (Input). D, Acetylation and methylation status of histones H3 and H4 after incubation with TSA. Cells were incubated with 1 μM TSA or vehicle for 24 hours and fixed by 1% PFA. Results were standardized using DNA before precipitation.