Assessment of hNIS expression in GLV-1h153-infected PANC-1 cells. a. Microarray analysis of cells infected with an MOI of 5.0 of GLV-1h153 yielded an almost 2000-fold increase by 6 hours and an almost 5000-fold increase by 24 hours in hNIS mRNA production as compared to noninfected control. b. PANC-1 cells were either mock infected or infected with GLV-1h68 at an MOI of 1.0 or infected with GLV-1h153 at an MOI of 1.0 or 5.0 for 24 hours. The hNIS protein was detected by Western blot analysis using monoclonal anti-hNIS antibody. Only GLV-1h153-infected cells expressed the hNIS protein, but cells either mock infected or infected with GLV-1h68 did not. The molecular weight marker bands (in kiloDaltons) are shown on the left. c. PANC-1 cells were mock infected or infected with GLV-1h68 or GLV-1h153 at a MOI of 1.0 for 24 hours. The hNIS protein was detected by immunofluorescence microscopy using monoclonal anti-hNIS antibody, which recognizes the intracellular domain of the protein. Mock- or GLV-1h68-infected cells (as demonstrated by GFP expression) did not express the hNIS protein, whereas the hNIS protein on the cell membrane of PANC-1 cells infected with GLV-1h153 was readily detectable.