Figure 5

Enhanced stimulation of MHC class II-restricted and non-classical MHC class I-restricted T cell lines by NP-loaded DC. (A-B) MHC class II-restricted CD4+ T cell lines: imDC incubated with: 100 μg/ml NP containing B5 peptide (▲); empty nanoparticles (▼); or 10 μg/ml B5 peptide (■) for one hour. (A) B5-reactive CD4+ T cells co-cultured with imDC. A 72-hour assay was performed, with ³H-thymidine added for the last 8 hours. (B) DC cultured for another 48 hours before co-culture with the responder T cells and a ³H-thymidine assay. (C) MHC class I-restricted CD8+ T cell lines: imDC incubated with 100/200 μg/ml empty nanoparticles (CNP), or with nanoparticles containing the peptide p42 (NP42) for one hour. Subsequently, p42-reactive CD8+ T cells were co-cultured with DC, and 48-hour later, IFN-gamma was measured by ELISA. Data is representative of 3 independent experiments; bars, SD. *, Significant differences (P < 0.05) between experimental and control cultures.