In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2

Table 2 Apoptosis induction by CTL007 in reconstruct with autologous WC007 CRC cells*

Lymphocytes	E:T	Total number of tumor cells mean ± SD/field (10 fields)	Number of apoptotic tumor cells mean ± SD/field (10 fields)	Percentage of apoptotic cells, mean ± SD/field (10 fields)
Experiment I
CTL007	1:1	80.5 ± 38.8	23.5 ± 8.7	31.7 ± 11^{c, d}
PHA blast	1:1	136.2 ± 54.8	15.9 ± 6.8	12.1 ± 3.9^c
No lymphocytes	NA	90.1 ± 3.5	7.1 ± 2.8	8.1 ± 3.5^d
Experiment II
CTL007	10:1	116.7 ± 19.4	94.8 ± 19	79.5 ± 6.7^{a, b}
PHA blast	10:1	118.3 ± 23.6	34.7 ± 11.8	27.5 ± 4.7^a
No lymphocytes	NA	121.1 ± 22.3	25.8 ± 10.7	21.3 ± 10.1^b

^a-d Values with the same symbol differ significantly from each other (Student's 2-sided t-test; p < 0.0001.
* Reconstructs were established with a separating collagen-fibroblast layer, CTL007 or autologous PHA blasts were added onto the top layer. Reconstructs were harvested either on day 3 (experiment I) or day 4 (experiment II) after adding T cells. Day of harvesting of reconstruct cultures was based on optimum constriction of the collagen gel of reconstruct cultures. The ratio of apoptotic tumor cells was determined by counting apoptotic nuclei and intact tumor cells in sections stained with H&E. Data represented are from two independent experiments.

ISSN: 1479-5876