Figure 3

A. CTL007 migrates toward CCL2 in Transwell. T cells were placed in the top chamber of the Transwell. CCL2 (10 ng/ml) in T cell medium, or T cell medium alone were added to the bottom of the Transwell (duplicate wells). After 90 min of culture, the number of migrated cells in the bottom chamber was counted under the microscope. Some T cell cultures were pre-incubated and treated with saturating concentration (10 μg/ml) of mouse anti-CCR2 Ab or mouse IgG as control. B. Expression of CCR2. T cells grown in log phase were incubated with either anti-CCR2 or anti-CXCR3 Ab (black or grey solid line) or mouse IgG control (dotted line) in RPMI 1640 with 5% human AB serum for 1 h at 4°C. After washing, FITC-labeled anti-mouse IgG was added. Expression of chemokine receptors was detected by flow cytometry.