Figure 3

MiR-205 is not involved in cellular proliferation or apoptosis of ESCC. Transfection of miR-205 precursor or anti-miR-205 inhibitor with sufficient concentrations substantially increased or decreased miR-205 expression levels in OE21 cells, respectively, assessed by quantitative RT-PCR (A). There were no significant differences between OE21 cells transfected with miR-205 precursor and anti-miR-205 inhibitor at the indicated concentrations in the optical densities of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assays (B). There were no significant differences in the percentages of apoptotic cells with morphological characteristics between the OE-21 cells transfected with miR-205 precursor and anti-miR-205 inhibitor at the indicated concentrations (C).