Figure 2

MiRNA-10a and miR-205 expression levels in various malignant cell types. Quantitative reverse transcriptase (RT)-PCR revealed that the miR-10a expression levels were decreased in ESCC cell lines (OE21, TE5, TE8, TE10, and TE11) compared to Het1A cells but the other cell lines (Caco2 and Jurkat) had more decreased expression (A). On the other hand, the miR-205 expression levels were exclusively increased in each ESCC cell line compared to those in any other malignant cell types examined and Het-1A cells (B). Northern blot analysis showed the intense miR-205 expression in OE21 cells despite its nominal expression in Het-1A cells (C).