Figure 3

Measurement of 2-PMPA in rat paw pads using LC-MS/MS - (A) Daughter-scan product ion spectrum of 2-PMPA. Monitoring was carried out at m/z 226.8 → 191.1 (B) Select rodent paw pad obtained 1 hour after 2-PMPA (100 mg/kg, i.p.) administration. Retention times for 2-PMPA and internal standard (temazepan) were approximately 1.3 and 2.0 min respectively. When rodent paw pads from untreated animals were used, only the internal standard peak was observed.