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Figure 5 | Journal of Translational Medicine

Figure 5

From: Shipping blood to a central laboratory in multicenter clinical trials: effect of ambient temperature on specimen temperature, and effects of temperature on mononuclear cell yield, viability and immunologic function

Figure 5

Mitogen and antigen-activated PBMC responses as detected by IFNgamma secretion in an ELIspot assay. After thawing from liquid nitrogen, PBMC were incubated 18 hours at 37°C with (A) PMA/ionomycin, (B) PHA or (C) CEF peptide pool and then tested for IFNg secretion by ELIspot assay. Results are presented as SFC per 200,000 PBMC for PMA and PHA. CEF SFC are adjusted for the percentage of CD8+ T cells and presented as SFC per 200,000 CD8 T cells. Each condition is compared to the control condition (arrows) as described in Figure 3. (*) p < 0.004.

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Journal of Translational Medicine

ISSN: 1479-5876

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