Figure 3

Transfection of miRNA-449a/b inhibitors and mimics and effects under hypoxia (original Figure 5). Primary human fibroblasts were transfected with miRNA-449a/b inhibitor or miRNA-449a/b mimics and were cultured under hypoxia. Inhibition strongly decreased miRNA-449a/b expression. Mimics showed an exaggerated effect on the miRNA-449a/b level. The mean, minimum and maximum of calculations relative to reference gene RNU48 compared with non-transfected cells (= control) and cells transfected with negative control siRNA only (= negative control) cultured under hypoxia are depicted. Results from 3 independent experiments are shown for cell line F-18, but were likewise demonstrable in M15D.