Figure 2

Microvessel density analysis of sorafenib treated tumors. Immunohistochemical analysis of CD31 (A-C) and CD34 (D-F) expression in vehicle (A, D), low dose (B, E) and high dose (C, F) at day3 are shown. The bar graph shows the average CD31 and CD34 expression in the vehicle (n = 3), conventional dose (n = 3), and high dose (n = 3) arms. As compared to vehicle treated tumors, the tumors exposed to conventional and high dose sorafenib had lower MVD (CD34 and CD31)(P < 0.01). There was a consistent trend for lower MVD in the high dose treatment as compared to the conventional dose treatment in both the CD34 and CD31 analyses.