Figure 3

CXC12/CXCR4 expression in Caco-2, HT-29 and SW480 cells as determined by (A) Q-RT-PCR for CXCL12 and CXCR4, (B) ELISA analysis for CXCL12 and (C) immunocytochemistry for CXCR4. (A) Q-RT-PCR data are expressed as mean +/- standard error of the mean (SEM), *P < 0.05. Fold increase above 1 indicates gene overexpression related to housekeeping gene B2M. (B) Detection of CXCL12 protein concentrations in pg/ml in cell culture supernatant of Caco-2, HT-29 and SW480 cells. Protein data are expressed as mean +/- SEM. (C) Detection of CXCR4 protein expression in representative cell culture slides of Caco-2, HT-29 and SW480 cells as assessed by immunocytochemical staining with CXCR4-specific antibodies.