Figure 4

Murine IL-10^-/- DCs are powerful Th17 inducers. a, b) Increased Th17 cells in IL-10^-/- mice. Mononuclear cells from unchallenged IL-10^+/+ and IL-10^-/- mouse organs were analyzed via FACS. a) Representative FACS plots. b) Results are expressed as the percentage of Th17 cells in CD4⁺ T cell populations ± SEM. 6 mice/group, *P < 0.05. c, d). IL-10^-/- spleen T cells were susceptible to Th17 polarization. IL-10^+/+ and IL-10^-/- spleen T cells were polarized with Th17-polarizing cytokines for 6 days. Th17 cells were analyzed by FACS. c) Results are expressed as the percentage of Th17 cells in CD4⁺ T cells. d) Supernatant from Th17 cultures was collected on day 3 and analyzed via ELISA for levels of IL-17. *P < 0.05, average of three experiments. e) IL-10^-/- DCs were potent Th17 inducers. IL-10^+/+ CD4⁺ T cells were cultured with IL-10^-/- or IL-10^+/+ splenic DCs in a ratio of 5:1 for 5 days. Th17 cells were analyzed by FACS. Results are expressed as the percentage of Th17 cells in CD4⁺ T cells. n = 6, *P < 0.05. Y axis showed SSC and the dot plots were gated on CD4⁺CD3⁺ T cells (a, c, e).