Figure 2

Relationships among IL-17, IL-1, and IL-10 in CD patients. a-c) IL-17, IL-10, and IL-1β message from fresh CD mononuclear lamina propria cells were quantified via real-time PCR. Chi-squared (χ²) test. P < 0.0001 for the following comparisons: IL-17 vs IL-10 (a), IL-1β vs IL-10 (b), and IL-17 vs IL-1β (c). n = 16. d) Crohn's DCs expressed high levels of IL-1β. Crohn's DCs and macrophages, and normal colon DCs were cultured for 40 hours with or without LPS. Normal control DCs were isolated from normal colon tissues at least 5 cm away from colon cancer. IL-1β protein was quantified via ELISA. n = 6. *, P < 0.005. e) Crohn's DCs induced high levels of T cell-derived IL-17 production through IL-1. CD4⁺ T cells were cultured for 48 hours with Crohn's myeloid DCs or macrophages in the presence anti-IL-1R. IL-17 protein was detected via ELISA in supernatants. n = 6. *, P < 0.005.