Molecular features of the complementarity determining region 3 motif of the T cell population and subsets in the blood of patients with chronic severe hepatitis B

Table 5 Representative amino acid sequences of monoclonal TCRBV families in CD8⁺ and CD8^- cells (depleted CD8⁺ cells) from CHSB patients.

Patients^a	Vbeta		CDR3	BJ		Ratio
89 (CD8⁺)	BV18	SAAYFCVSS	RTGDTEA	FFGQGTRLTVVED	1.1	11/11
133 (CD8⁺)	BV11	SQYLCASS	AGEL	FFGEGSRLTVLED	2.2	11/20
115 (CD8⁺)	BV11	SQYLCATG	VYNEQ	FFGPGTRLTVLED	2.1	14/16
84 (CD8⁺)	BV11	SQYLCATG	VYNEQ	FFGPGTRLTVLED	2.1	27/27
119 (CD8^-)	BV11	SQYLCASS	AGEL	FFGEGSRLTVLED	2.2	14/22
89 (CD8^-)	BV11	SQYLCATG	VYNEQ	FFGPGTRLTVLED	2.1	18/20
84 (CD8^-)	BV11	SQYLCATG	VYNEQ	FFGPGTRLTVLED	2.1	17/20
89 (CD8⁺)	BV5.2	SALYLCASS	LTAGAYTGEL	FFGEGSRLTVLED	2.2	11/29

When the GMSP displayed a single peak (Figure 3), the cDNA of corresponding TCRBV family was re-amplified and PCR product was sequenced after cloning. The amino acid sequences of the max rate of TCRBV families are shown. ^a The cell subset for analysis in brackets.

ISSN: 1479-5876