Molecular features of the complementarity determining region 3 motif of the T cell population and subsets in the blood of patients with chronic severe hepatitis B

Table 4 Representative amino acid sequences of monoclonal TCRBV families in PBMCs from CSHB patients.

Patients	Vbeta		CDR3	BJ		Ratio
69	BV23	SALYFCASS	VEGGNTI	YFGEGSWLTVVED	1.3	21/21
90	BV22	SAMYFCASS	DLGVAQ	YFGPGTRLTVTED	2.7	9/20
75	BV22	SAMYFCASS	DLGVAQ	YFGPGTRLTVTED	2.7	4/18
90	BV18	SAAYFCASS	RTGDTEA	FFGQGTRLTVVED	1.1	21/21
75	BV18	SAAYFCASS	RTGDTEA	FFGQGTRLTVVED	1.1	21/21
105	BV18	SAAYFCASS	RTGDTEA	FFGQGTRLTVVED	1.1	21/21
107	BV11	SQYLCASS	AGEL	FFGEGSRLTVLED	2.2	5/21
131	BV11	SQYLCASS	AGEL	FFGEGSRLTVLED	2.2	9/20
138	BV11	SQYLCASS	AGEL	FFGEGSRLTVLE	2.2	9/18
172	BV11	SQYLCASS	AGEL	FFGEGSRLTVLED	2.2	3/21
172	BV11	SQYLCATG	VYNEQ	FFGPGTRLTVLED	2.1	4/21
75	BV11	SQYLCATG	VYNEQ	FFGPGTRLTVLED	2.1	7/8
83	BV11	SQYLCATG	VYNEQ	FFGPGTRLTVLED	2.1	20/20
71	BV11	SQYLCATG	VYNEQ	FFGPGTRLTVLED	2.1	21/21
69	BV9	SAVYFCASS	LQAGRGEQ	FFGPGTRLTVLED	2.1	12/20
172	BV7	SALYLCASS	QDSVTTGAQ	YFGPGTRLLVLED	2.5	15/21
90	BV6	SAVYLCASS	LAWEEQETQ	YFGPGTRLLVLED	2.5	21/21
81	BV5.2	SALYLCASS	LTAGAYTGEL	FFGEGSRLTVLED	2.2	10/22
2	BV5.1	SALYLCASS	LEWGASYEQ	YFGPGTRLTVTED	2.7	9/20

When the GMSP displayed a single peak (Figure 2), the cDNA of corresponding TCRBV family was re-amplified and PCR product was sequenced after cloning. The amino acid sequences of the max rate of TCRBV families are shown.

ISSN: 1479-5876