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Table 1 Primer sequences used in this research.

From: Differentiation of breast cancer stem cells by knockdown of CD44: promising differentiation therapy

Gene

Sequence

GeXP PCR product (bp)

Multiplex 1: 17 genes (+ GAPDH and KanR)

Bcl-2 (B-cell lymphoma 2)

F: AGGTGACACTATAGAATATGGAACTGTACGGCCCCAGCA

140

[Genebank:NM_000633]

R: GTACGACTCACTATAGGGAAGGGTGATGCAAGCTCCCACCA

 

Fos (FBJ murine osteosarcoma viral oncogene homolog)

F: AGGTGACACTATAGAATAGTTATAAAAGCAGTGGCTGCGGC

153

[Genebank: NM_005252]

R: GTACGACTCACTATAGGGAAGCACGGTCACTGCTCGTTCG

 

ICAM1 (intercellular adhesion molecule 1)

F: AGGTGACACTATAGAATAGTGCTATTCAAACTGCCCTGA

169

[Genebank:NM_000201]

R: GTACGACTCACTATAGGGAGCGTAGGGTAAGGTTCTTGC

 

CCND1 (cyclin D1)

F: AGGTGACACTATAGAATAGCATGTTCGTGGCCTCTAAG

177

[Genebank: NM_053056]

R: GTACGACTCACTATAGGGACAGGTTCCACTTGAGCTTGTT

 

MMP7 (matrix metalloproteinase-7)

F: AGGTGACACTATAGAATATTGGCTTTGCGCGAGGAGCT

181

[Genebank:NM_002423]

R:GTACGACTCACTATAGGGACTGCTACCATCCGTCCAGCGT

 

Myc (V-myc myelocytomatosis viral oncogene homolog (avian))

F: AGGTGACACTATAGAATACTCTCCTTGCAGCTGCTTAGA

187

[Genebank:NM_002467]

R: GTACGACTCACTATAGGGACCTCGTCGCAGTAGAAATACG

 

PRKCE (Protein kinase C epsilon type)

F: AGGTGACACTATAGAATAAAGGTCCCTACCTTCTGCGA

197

[Genebank:NM_005400]

R: GTACGACTCACTATAGGGACCAGTACTTTGGCGATTCCT

 

TP53 (Cellular Tumor Antigen p53)

F: AGGTGACACTATAGAATATTCCCTGGATTGGCAGCCAGACTG

201.5

[Genebank:NM_000546]

R: GTACGACTCACTATAGGGATCCATTGCTTGGGACGGCAAGG

 

VCAM1 (vascular cell adhesion molecule 1)

F: AGGTGACACTATAGAATAAGGTGACGAATGAGGGGACCACATC

215.4

[Genebank:NM_001078]

R: GTACGACTCACTATAGGGAAGCCTCCAGAGGGCCACTCAAA

 

IL4R (IL-4 receptor)

F: AGGTGACACTATAGAATAAAGTGGCACAACTCCTACAGG

231

[Genebank:NM_000418]

R: GTACGACTCACTATAGGGACCCTGAGCATCCTGGATTATT

 

PTCH1 (Protein patched homolog 1)

F: AGGTGACACTATAGAATAGCCTTCGCTCTGGAGCAGATTT

240

[Genebank:NM_000264]

R: GTACGACTCACTATAGGGAGTTGGTCTCGAGGTTCGCTGCTT

 

HSPB1(heat shock 27 kDa protein 1)

F: AGGTGACACTATAGAATAGCACACTGACCGTGGAGGCC

241

[Genebank: NM_001540]

R: GTACGACTCACTATAGGGAGAACACACAGGTGGCGGGGG

 

PTGS2 (prostaglandin-endoperoxide synthase 2)

F: AGGTGACACTATAGAATACAGCTCCACAGCCAGACGCC

249

[Genebank: NM_000963]

R: GTACGACTCACTATAGGGATCCTGTCCGGGTACAATCGCACT

 

HSF1 (Heat shock factor protein 1)

F: AGGTGACACTATAGAATAGCCTTCCTGACCAAGCTGTGGACC

271

[Genebank:NM_005526]

R: GTACGACTCACTATAGGGATCTCTCTGGCTTGACCAGGCCG

 

GAPDH (Glyceraldehyde 3-phosphate dehydrogenase)

F: AGGTGACACTATAGAATAAAGGTGAAGGTCGGAGTCAA

277.2

[Genebank:NM_002046]

R: GTACGACTCACTATAGGGAGATCTCGCTCCTGGAAGATG

 

LEF1 (Lymphoid enhancer-binding factor-1)

F: AGGTGACACTATAGAATAGGTGCAGCCATCCCATGCGGT

290.2

[Genebank:NM_016269]

R: GTACGACTCACTATAGGGAAGGGTTGCCTGAATCCACCCGTG

 

TCF7 (Transcription factor 7)

F: AGGTGACACTATAGAATAGGCGAGGAGCAGGACGACAAGAG

305

[Genebank:NM_003202]

R: GTACGACTCACTATAGGGATTTGTACATGCCGCTGGTGCAC

 

FASN (Fatty acid synthase)

F: AGGTGACACTATAGAATAGAGTCGGAGAACTTGCAGGAGT

307

[Genebank:NM_004104]

R: GTACGACTCACTATAGGGAGTGTGTTCCTCGGAGTGAATCT

 

KanR (Kanamycin resistance)

F: AGGTGACACTATAGAATAATCATCAGCATTGCATTCGATTCCTGTTTG

325.4

 

R: GTACGACTCACTATAGGGAATTCCGACTCGTCCAACATC

 

Multiplex 1: 5 genes (+ GAPDH and KanR)

Muc-1 (Mucin 1, transmembrane, transcript variant 1)

F: AGGTGACACTATAGAATAGACGTCAGCGTGAGTGATGT

177.7

[Genebank:NM_002456]

R: GTACGACTCACTATAGGGAGACAGCCAAGGCAATGAGAT

 

Cyclin E2 (Cyclin E2 (CCNE2), transcript variant 3)

F: AGGTGACACTATAGAATAGAGCCCGAAGAGCACTGAAAAACC

194.6

[Genebank:NM_004702]

R: GTACGACTCACTATAGGGAGAGGAATTGGCTAGGGCAATCAA

 

EGFR (EGF receptor, transcript variant 1)

F: AGGTGACACTATAGAATAGAAAGGCAGCCACCAAATTA

208.4

[Genebank:NM_005228]

R: GTACGACTCACTATAGGGAACTATCCTCCGTGGTCATGC

 

Myc (V-myc myelocytomatosis viral oncogene homolog (avian))

F: AGGTGACACTATAGAATAGAGCAACGTCTCCACACATCAGCAC

217

[Genebank:NM_002467]

R: GTACGACTCACTATAGGGAGAGTTTTGTGTGTTCGCCTCTTGAC

 

Cyclin D1 (G1/S-specific cyclin-D1)

F: AGGTGACACTATAGAATACGTGGCCTCTAAGATGAAGG

252.5

[Genebank:BC001501]

R: GTACGACTCACTATAGGGATGCGGATGATCTGTTTGTTC

 

GAPDH (Glyceraldehyde 3-phosphate dehydrogenase)

F: AGGTGACACTATAGAATAAAGGTGAAGGTCGGAGTCAA

277.2

[Genebank:NM_002046]

R: GTACGACTCACTATAGGGAGATCTCGCTCCTGGAAGATG

 

KanR (Kanamycin resistance)

F: AGGTGACACTATAGAATAATCATCAGCATTGCATTCGATTCCTGTTTG

325.4

 

R: GTACGACTCACTATAGGGAATTCCGACTCGTCCAACATC

 
  1. F: Forward; R: Reverse.