From: Differentiation of breast cancer stem cells by knockdown of CD44: promising differentiation therapy
Gene | Sequence | GeXP PCR product (bp) |
---|---|---|
Multiplex 1: 17 genes (+ GAPDH and KanR) | ||
Bcl-2 (B-cell lymphoma 2) | F: AGGTGACACTATAGAATATGGAACTGTACGGCCCCAGCA | 140 |
[Genebank:NM_000633] | R: GTACGACTCACTATAGGGAAGGGTGATGCAAGCTCCCACCA | Â |
Fos (FBJ murine osteosarcoma viral oncogene homolog) | F: AGGTGACACTATAGAATAGTTATAAAAGCAGTGGCTGCGGC | 153 |
[Genebank: NM_005252] | R: GTACGACTCACTATAGGGAAGCACGGTCACTGCTCGTTCG | Â |
ICAM1 (intercellular adhesion molecule 1) | F: AGGTGACACTATAGAATAGTGCTATTCAAACTGCCCTGA | 169 |
[Genebank:NM_000201] | R: GTACGACTCACTATAGGGAGCGTAGGGTAAGGTTCTTGC | Â |
CCND1 (cyclin D1) | F: AGGTGACACTATAGAATAGCATGTTCGTGGCCTCTAAG | 177 |
[Genebank: NM_053056] | R: GTACGACTCACTATAGGGACAGGTTCCACTTGAGCTTGTT | Â |
MMP7 (matrix metalloproteinase-7) | F: AGGTGACACTATAGAATATTGGCTTTGCGCGAGGAGCT | 181 |
[Genebank:NM_002423] | R:GTACGACTCACTATAGGGACTGCTACCATCCGTCCAGCGT | Â |
Myc (V-myc myelocytomatosis viral oncogene homolog (avian)) | F: AGGTGACACTATAGAATACTCTCCTTGCAGCTGCTTAGA | 187 |
[Genebank:NM_002467] | R: GTACGACTCACTATAGGGACCTCGTCGCAGTAGAAATACG | Â |
PRKCE (Protein kinase C epsilon type) | F: AGGTGACACTATAGAATAAAGGTCCCTACCTTCTGCGA | 197 |
[Genebank:NM_005400] | R: GTACGACTCACTATAGGGACCAGTACTTTGGCGATTCCT | Â |
TP53 (Cellular Tumor Antigen p53) | F: AGGTGACACTATAGAATATTCCCTGGATTGGCAGCCAGACTG | 201.5 |
[Genebank:NM_000546] | R: GTACGACTCACTATAGGGATCCATTGCTTGGGACGGCAAGG | Â |
VCAM1 (vascular cell adhesion molecule 1) | F: AGGTGACACTATAGAATAAGGTGACGAATGAGGGGACCACATC | 215.4 |
[Genebank:NM_001078] | R: GTACGACTCACTATAGGGAAGCCTCCAGAGGGCCACTCAAA | Â |
IL4R (IL-4 receptor) | F: AGGTGACACTATAGAATAAAGTGGCACAACTCCTACAGG | 231 |
[Genebank:NM_000418] | R: GTACGACTCACTATAGGGACCCTGAGCATCCTGGATTATT | Â |
PTCH1 (Protein patched homolog 1) | F: AGGTGACACTATAGAATAGCCTTCGCTCTGGAGCAGATTT | 240 |
[Genebank:NM_000264] | R: GTACGACTCACTATAGGGAGTTGGTCTCGAGGTTCGCTGCTT | Â |
HSPB1(heat shock 27 kDa protein 1) | F: AGGTGACACTATAGAATAGCACACTGACCGTGGAGGCC | 241 |
[Genebank: NM_001540] | R: GTACGACTCACTATAGGGAGAACACACAGGTGGCGGGGG | Â |
PTGS2 (prostaglandin-endoperoxide synthase 2) | F: AGGTGACACTATAGAATACAGCTCCACAGCCAGACGCC | 249 |
[Genebank: NM_000963] | R: GTACGACTCACTATAGGGATCCTGTCCGGGTACAATCGCACT | Â |
HSF1 (Heat shock factor protein 1) | F: AGGTGACACTATAGAATAGCCTTCCTGACCAAGCTGTGGACC | 271 |
[Genebank:NM_005526] | R: GTACGACTCACTATAGGGATCTCTCTGGCTTGACCAGGCCG | Â |
GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) | F: AGGTGACACTATAGAATAAAGGTGAAGGTCGGAGTCAA | 277.2 |
[Genebank:NM_002046] | R: GTACGACTCACTATAGGGAGATCTCGCTCCTGGAAGATG | Â |
LEF1 (Lymphoid enhancer-binding factor-1) | F: AGGTGACACTATAGAATAGGTGCAGCCATCCCATGCGGT | 290.2 |
[Genebank:NM_016269] | R: GTACGACTCACTATAGGGAAGGGTTGCCTGAATCCACCCGTG | Â |
TCF7 (Transcription factor 7) | F: AGGTGACACTATAGAATAGGCGAGGAGCAGGACGACAAGAG | 305 |
[Genebank:NM_003202] | R: GTACGACTCACTATAGGGATTTGTACATGCCGCTGGTGCAC | Â |
FASN (Fatty acid synthase) | F: AGGTGACACTATAGAATAGAGTCGGAGAACTTGCAGGAGT | 307 |
[Genebank:NM_004104] | R: GTACGACTCACTATAGGGAGTGTGTTCCTCGGAGTGAATCT | Â |
KanR (Kanamycin resistance) | F: AGGTGACACTATAGAATAATCATCAGCATTGCATTCGATTCCTGTTTG | 325.4 |
 | R: GTACGACTCACTATAGGGAATTCCGACTCGTCCAACATC |  |
Multiplex 1: 5 genes (+ GAPDH and KanR) | ||
Muc-1 (Mucin 1, transmembrane, transcript variant 1) | F: AGGTGACACTATAGAATAGACGTCAGCGTGAGTGATGT | 177.7 |
[Genebank:NM_002456] | R: GTACGACTCACTATAGGGAGACAGCCAAGGCAATGAGAT | Â |
Cyclin E2 (Cyclin E2 (CCNE2), transcript variant 3) | F: AGGTGACACTATAGAATAGAGCCCGAAGAGCACTGAAAAACC | 194.6 |
[Genebank:NM_004702] | R: GTACGACTCACTATAGGGAGAGGAATTGGCTAGGGCAATCAA | Â |
EGFR (EGF receptor, transcript variant 1) | F: AGGTGACACTATAGAATAGAAAGGCAGCCACCAAATTA | 208.4 |
[Genebank:NM_005228] | R: GTACGACTCACTATAGGGAACTATCCTCCGTGGTCATGC | Â |
Myc (V-myc myelocytomatosis viral oncogene homolog (avian)) | F: AGGTGACACTATAGAATAGAGCAACGTCTCCACACATCAGCAC | 217 |
[Genebank:NM_002467] | R: GTACGACTCACTATAGGGAGAGTTTTGTGTGTTCGCCTCTTGAC | Â |
Cyclin D1 (G1/S-specific cyclin-D1) | F: AGGTGACACTATAGAATACGTGGCCTCTAAGATGAAGG | 252.5 |
[Genebank:BC001501] | R: GTACGACTCACTATAGGGATGCGGATGATCTGTTTGTTC | Â |
GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) | F: AGGTGACACTATAGAATAAAGGTGAAGGTCGGAGTCAA | 277.2 |
[Genebank:NM_002046] | R: GTACGACTCACTATAGGGAGATCTCGCTCCTGGAAGATG | Â |
KanR (Kanamycin resistance) | F: AGGTGACACTATAGAATAATCATCAGCATTGCATTCGATTCCTGTTTG | 325.4 |
 | R: GTACGACTCACTATAGGGAATTCCGACTCGTCCAACATC |  |