Figure 3
From: Differentiation of breast cancer stem cells by knockdown of CD44: promising differentiation therapy
CD44 expression before (A, B, C and D) and after CD44 knockdown by shRNA (E, F, G and H) combined with selection using puromycin dihydrochloride for 1 week. Cells were evaluated by immunocytochemistry after staining with anti-CD44 and fluorescein-isothiocyanate (FITC)-conjugated secondary antibody and were observed under a monochromatic fluorescence microscope (Carl Zeiss, Oberkochen, Germany) with white light, Hoechst 33342 and a FITC filter (A and E, B and F, C and G, respectively) and by flow cytometry after staining with anti-CD44-FITC (D and H).