Figure 8

Schematic diagram of ECL-based assay for human IA. A) Non-specifically binding material is removed from 1:10 diluted serum or plasma by incubation with streptavidin coated paramagnetic beads (SA-beads), followed by magnetic removal to yield a clarified sample. B) Biotinylated-insulin is incubated with the clarified sample and binds to IA in solution. C) Bound IA-biotinylated insulin complexes are precipitated with SA-beads. D) Ruthenium-labeled secondary goat anti-human IgG antibody binds to IA-bead complexes and is detected on the M1MR Analyzer.