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Figure 4 | Journal of Translational Medicine

Figure 4

From: Antibodies against insulin measured by electrochemiluminescence predicts insulitis severity and disease onset in non-obese diabetic mice and can distinguish human type 1 diabetes status

Figure 4

Insulitis index correlates with murine IAA ECL values. NOD mice were followed from 4 weeks up to 20 weeks of age. Serum samples were collected weekly and IAA levels were measured by our murine IAA ECL assay. Mice were euthanized at various ages and pancreatic tissue was extracted and stained with H&E. A) Insulitis was scored as depicted in the H&E stained tissue sections. The formula for calculating insulitis index is indicated below. B) Insulitis index was correlated with the IAA values at the age the pancreas was removed for histology (final measurement). C) Insulitis index was correlated with the maximum IAA values measured for each mouse during the study. Data were plotted for NOD pancreas tissues obtained from mice at 5-20 weeks and 5-10 weeks of age. IAA value is calculated as the average signal from triplicate wells divided by the background signal from triplicate wells with beads alone. The Spearman's correlation coefficient (rs) and the corresponding p-value for each age group are displayed on the graphs. The trends showing highest correlation (rs values) when restricting the analysis to younger mice is graphed (bottom panels), where the x-axis is the upper age limit (in weeks) of the mice included in the analysis.

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