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Figure 1 | Journal of Translational Medicine

Figure 1

From: Antibodies against insulin measured by electrochemiluminescence predicts insulitis severity and disease onset in non-obese diabetic mice and can distinguish human type 1 diabetes status

Figure 1

Protocol for murine IAA ECL assay. A) Overview of the murine IAA ECL procedure. B) IAA in serum is precipitated by biotinylated-insulin conjugated to streptavidin-coated magnetic beads, while a ruthenium (Ru)-tagged secondary antibody detects the bound IgG autoantibodies. 2° = secondary.

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Journal of Translational Medicine

ISSN: 1479-5876

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