Figure 1
IHC analysis of TMA of non-GIST STS representing different expressions of markers belonging to PI3K/Akt pathway in tumor cells. A, Leiomyosarcoma, histological grade I, PTEN, negative staining, score 0; B, Pleomorphic liposarcoma, histological grade III, p-Akt Thr308, weak nuclear staining, score 1; C, Undifferentiated pleomorphic sarcoma, histological grade III, PI3K, moderate cytoplasmic staining; score 2; D, Dedifferentiated liposarcoma, histological grade II, p-Akt Ser473, strong both nuclear and cytoplasmic staining, score 3. All calibration bars correspond to 100 μm in the overview images (objective 10×) and 50 μm in the pictures taken at high magnification (objective 40×). Abbreviations: IHC, immunohistochemistry; TMA, tissue microarray; non-GIST STS, non gastro-intestinal stromal tumor soft-tissue sarcoma; PTEN, phosphatase and tensin homolog; p-Akt Thr308, Akt phosphorylated at threonin 308; PI3K, phosphoinositide 3-kinase; p-Akt Ser473, Akt phosphorylated at serin 473.