No significant difference in the percentage recovery of viable lysate-loaded DCs cryopreserved in infusible cryomedia or 90% human AB serum with 10% DMSO was observed post-thawed. Mature lysate-loaded DCs were frozen at 5 × 106 cells per ml in infusible cryomedia or 90% human AB serum plus 10% DMSO, and thawed 24 h later to determine the % of viable cell recovery. The % of viable DCs was calculated by Trypan blue exclusion using the following formula: (total number of live non-Trypan blue DCs in all the four big squares of the Bürker chamber total number of live and dead DCs counted in the four big squares of the Bürker chamber) × 100%. Data is the mean of 3 independent experiments (± SEM). P = 0.6 (NS; no significant difference in the viability of thawed DCs was observed when comparing the use of infusible cryomedia and 90% human AB serum with 10% DMSO) [unpaired Student's t test].