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Table 3 DC viability following cryopreservation in two different cryomedia.

From: Optimizing parameters for clinical-scale production of high IL-12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate

Cryopreservation method % viable DC (± SEM)  
Infusible cryomedia 92.9 ± 2.57 P = 0.6 (NS)
90% human AB serum + 10% DMSO 86.5 ± 0.55 P = 0.6 (NS)
  1. No significant difference in the percentage recovery of viable lysate-loaded DCs cryopreserved in infusible cryomedia or 90% human AB serum with 10% DMSO was observed post-thawed. Mature lysate-loaded DCs were frozen at 5 × 106 cells per ml in infusible cryomedia or 90% human AB serum plus 10% DMSO, and thawed 24 h later to determine the % of viable cell recovery. The % of viable DCs was calculated by Trypan blue exclusion using the following formula: (total number of live non-Trypan blue DCs in all the four big squares of the Bürker chamber total number of live and dead DCs counted in the four big squares of the Bürker chamber) × 100%. Data is the mean of 3 independent experiments (± SEM). P = 0.6 (NS; no significant difference in the viability of thawed DCs was observed when comparing the use of infusible cryomedia and 90% human AB serum with 10% DMSO) [unpaired Student's t test].