Time-course of IL-12p70 production by DCs. IL-12p70 was detected from fresh DCs from 6 h post LPS and IFN-γ stimulation and peaked at 16 h. Fresh replated DCs and thawed replated DCs continued to produce IL-12p70 albeit at a lower level than fresh DCs. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in duplicates in research-scale Nunclon™Δ Surface T25 cm2 flasks ± SEM. IL-12p70 concentration is expressed as pg/ml.