Comparison of the effect of Nunclon™Δ Surface, Corning® cell-culture surface and Corning® ultra-low attachment surface on DC phenotype and differentiation. DC were cultured with CellGenix DC media containing 2% human AB serum in T25 cm2 flasks that had the same surface properties as one of the three commercially available cell factories - Nunclon™Δ Surface 1-tray Cell Factories, Corning® CellSTACK® culture chambers or Corning® cell culture ultra-low attachment CellSTACK® chamber. DC phenotype was evaluated following lysate-loading and maturation for 16 h with LPS and IFN-γ. (A) DCs cultured in Nunclon™Δ Surface flasks (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) exhibited an overall favorable immunophenotype with significantly higher DC-LAMP (**P = 0.003) and lower CD14 (*P = 0.01) when compared to DCs cultured in Corning® cell-culture surface flasks (surrogate of Corning® CellSTACK® culture chambers). Data were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures ± SEM. (B) DCs that were cultured in Corning® ultra-low attachment surface (surrogate of Corning® ultra-low attachment cell factory chambers) expressed significantly lower CD86 (**P = 0.011) and HLA-DR (***P = 0.0004) following lysate-loading and maturation when compared to DCs cultured in Nunclon™Δ Surface (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) and treated the same way. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in research-scale cultures ± SEM. P values were determined with unpaired Student's t test. * denoted that P values were highly significant.