Figure 1
Comparison of the phenotype and IL-12p70 production of mature HOCl-oxidized lysate-loaded DCs generated in human AB serum-free or serum-containing AIM-V and CellGenix DC media, and supplementation with different concentrations of GM-CSF and IL-4. (A-B) DCs cultured in CellGenix DC media containing 2% human AB serum displayed a desirable phenotype with the highest level of DC-LAMP (*P = 0.005; one-way ANOVA) when compared to DCs cultured in other media conditions, and significantly higher CD86 (*P = 0.05) and lower CD14 (*P = 0.04) when compared to DCs cultured in serum-free CellGenix DC media. (C) DCs generated in CellGenix DC media plus 2% human AB serum produced slightly, though not significantly, higher amount of IL-12p70 compared to DCs generated in other media conditions (P = 0.2; one-way ANOVA). (D) 500 IU/ml recombinant GM-CSF and 250 IU/ml recombinant IL-4 were sufficient concentrations for generating a favorable immature DC phenotype with low CD14, and intermediate HLA-DR and CD1c. All the data presented were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures in Nunclon™Δ Surface T25 cm2 flasks ± standard error of the mean (SEM). P values were determined with unpaired Student's t test unless otherwise stated. * denoted that P values were significant.