Figure 1

Dose dependent inhibition of HCVpp of 3a genotype. HCVpp was produced in HEK 293 T cells and collected in media after filtration in 0.45 micron filter.(a) CD81 monoclonal antibody were incubated with Huh-7 cells at 37°C for 1 h. (b) HCV E2 monoclonal antibody incubated with HCV Pseudo particles at 37°C for 1 hr. After 1 hrs Huh-7 cells were infected with pseudo particle of HCV 3a genotype in the presence and absence of different concentrations of CD81 and HCV E2 antibody and incubated for 3 hrs. After 24 hrs cells were lysed and luciferase activity was determined by using a luminometer. Luciferase activity is not reported as an absolute value, but is calculated relative to the 'no drug' condition and reported on the y-axis as a percentage. Results are represented as the average and standard error for three independent experiments. P value > 0.05 vs control was considered as statistically significant.