Figure 2

Up-regulated expression of NY-ESO-1 in human glioma-derived cell lines and primary cell lines treated with decitabine. A) Representative data for established glioma cell lines; B) primary glioblastoma cells obtained from surgical resection; or C) normal human astrocytes (NHA) and a NY-ESO-1⁺ human melanoma line, 624.38. Only decitabine-treated glioma cells demonstrated expression of NY-ESO-1. D) Using SYBR green DNA labeling and quantitative PCR primers specific for NYESO-1 and GAPDH, the cDNA was amplified using a real-time PCR protocol. The relative fold change in gene expression is graphed showing an increase average fold change in treated T98G and glioblastoma #1 cells compared to untreated (***p = 0.0001 and **p = 0.007). Similar results were seen in three replicate experiments.