Figure 1

Treatment of human glioma with decitabine up-regulates cancer-testes antigens and MHC I. A) Total RNA was isolated from U-251MG glioma cells treated with decitabine or vehicle control and subjected to global gene expression classification using Affymetrix human U133 Plus 2.0 microarray chips. B) Decitabine-treated and untreated U-251MG glioma cells were stained with α-HLA-A, B, C and α-HLA-A2. Representative flow cytometric analysis data are shown here. Similar results were seen in two independent experiments.