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Figure 5 | Journal of Translational Medicine

Figure 5

From: Construction and characterisation of a recombinant fowlpox virus that expresses the human papilloma virus L1 protein

Figure 5

Expression of HPV-L1 and GFP transcripts over time using real-time PCR in restrictive mammalian cells. After infecting Vero cells with 2 PFU/cell of the FPL1 recombinant, quantitative expression of the HPV-L1 and GFP transgenes was evaluated every 3 days for 22 days. The maximum expression level for both HPV-L1 and GFP were reached on day 7 p.i., and GFP mRNAs were always highly expressed. The copy number of HPV-L1/GFP mRNAs, normalised to the RPS7 endogenous housekeeping transcripts, refers to the N-fold increase vs control mRNA at each time point. The data are expressed as means (┬▒standard deviation) from two independent analyses.

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